Interweaving mathematical modelling with quantitative experimental techniques
Microfluidic technologies
Microdroplets: Ultra-miniaturised test-tubes for single-cell encapsulation
Miniaturized screening in nanoliter droplets using droplet-on-demand technologies
Microdroplets: Ultra-miniaturised test-tubes for single-cell encapsulation
Bacterial single-cell microfluidics: bottom-up approaches to study AMR
The LSI hosts a number of experimentalists working with microfluidic technologies for quantitative biology and biosensing in various research areas. An example of our multi-disciplinary ethos in action is the combination of microfluidics experiments, led by Dr Jehangir Cama and Dr Stefano Pagliara, with mathematical modelling developed by Dr. Voliotis and Prof. Tsaneva-Atanasova, to tackle the challenge of quantifying antibiotic accumulation in individual Gram-negative bacteria - read article.
These studies are of great importance in the fight against antimicrobial resistance. The combination of mathematical modelling and microfluidics provided deeper insights into the process of drug accumulation in these microbes than could have been obtained by either approach in isolation.
Current projects include:
- Real time integration of simulation and control models with genetic networks in thousands of individual bacteria. Led by Dr Remy Chait.
- Optimising antibiotic treatment regimes, one bacterial cell at a time. Led by Dr Tobias Bergmiller.
- High-throughput screening of antimicrobial libraries using real-time label-free imaging of microfluidic droplets. Led by Dr Fabrice Gielen.