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Dr Fabrice Gielen

Dr Fabrice Gielen

Research Fellow

 7457

 +44 (0)1392 727457

 Living Systems Institute T04.15

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

Overview

I hold an MEng degree in micro and nanotechnology for integrated systems and an MRes degree in protein and membrane chemical biology. My PhD at Imperial College London in the laboratory of Prof. Joshua Edel focussed on the study of cellular membrane dynamics by developing microfluidic cell trapping platforms using dielectrophoresis and high-resolution fluorescence spectroscopy.

I joined Prof. Florian Hollfelder’s lab as a post-doc in 2011 with a view to applying microfluidic tools to important biological questions such as the interrogation of protein-protein interactions, the evolution and discovery of enzymes (e.g. from the metagenome), or the discovery of novel small molecule drugs.

I am also co-founder of Drop-Tech Ltd, a start-up that develops droplet-on-demand products (e.g. the Mitos Dropix, sold by Dolomite Microfluidics).

View my profile on the Physics and Astronomy website.

Qualifications

2008-2011 Ph.D. in Chemistry, Imperial College London, UK.
2007-2008 MRes in protein membrane chemical biology, Imperial College London, UK    
2004-2006 MEng. in Micro and Nanotechnology for Integrated Systems, Phelma, Grenoble, FR

Research

Research interests

The trend towards ever faster, cheaper and more efficient ways of discovering drugs or catalysts has been concomitant with miniaturization of assay volumes. One direction of research is the encapsulation of reagents with volumes down to picoliters using water-in-oil microemulsions.

My research focuses on the development of high-throughput screening platforms for molecular and cellular assays with special emphasis on drug screening and directed evolution. We use droplet microfluidics to rapidly encapsulate thousands to millions of (single) cells before rapidly identifying improved phenotypes with bespoke high-throughput optical tools.

Research grants

  • 2017 Royal Society Research Grant
    Identification and isolation of single cells in microfluidic droplets

Publications

Key publications | Publications by category | Publications by year

Publications by category


Journal articles

Anagnostidis V, Sherlock B, Metz J, Mair P, Hollfelder F, Gielen F (In Press). Deep learning guided image-based droplet sorting for on-demand selection. and analysis of single cells and 3D cell cultures.  Abstract.  Author URL.  Full text.
Buryska T, Vasina M, Gielen F, Vanacek P, van Vliet L, Jezek J, Pilat Z, Zemanek P, Damborsky J, Hollfelder F, et al (2019). Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics. Anal Chem, 91(15), 10008-10015. Abstract.  Author URL.  Full text.
Kleine-Brüggeney H, van Vliet LD, Mulas C, Gielen F, Agley CC, Silva JCR, Smith A, Chalut K, Hollfelder F (2019). Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation. Small, 15(5). Abstract.  Full text.
Gielen F, Colin P-Y, Mair P, Hollfelder F (2018). Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics. Methods Mol Biol, 1685, 297-309. Abstract.  Author URL.  Full text.
Mair P, Gielen F, Hollfelder F (2017). Exploring sequence space in search of functional enzymes using microfluidic droplets. Curr Opin Chem Biol, 37, 137-144. Abstract.  Author URL.  Full text.
Gielen F, Butz M, Rees EJ, Erdelyi M, Moschetti T, Hyvonen M, Edel JB, Kaminski CF, Hollfelder F (2017). Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets. ANALYTICAL CHEMISTRY, 89(2), 1092-1101. Author URL.
Gielen F, Hours R, Emond S, Fischlechner M, Schell U, Hollfelder F (2016). Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS). Proc Natl Acad Sci U S A, 113(47), E7383-E7389. Abstract.  Author URL.  Full text.
Chen W, Avezov E, Schlachter SC, Gielen F, Laine RF, Harding HP, Hollfelder F, Ron D, Kaminski CF (2015). A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth. Biophys J, 108(5), 999-1002. Abstract.  Author URL.
Gielen F, Buryska T, Van Vliet L, Butz M, Damborsky J, Prokop Z, Hollfelder F (2015). Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets. Anal Chem, 87(1), 624-632. Abstract.  Author URL.
Colin P-Y, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvönen M, Morgavi DP, Janssen DB, Hollfelder F, et al (2015). Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nat Commun, 6 Abstract.  Author URL.
Gielen F, van Vliet L, Koprowski BT, Devenish SRA, Fischlechner M, Edel JB, Niu X, deMello AJ, Hollfelder F (2013). A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition. Anal Chem, 85(9), 4761-4769. Abstract.  Author URL.
Hassan SU, Gielen F, Niu X, Edel JB (2012). Controlled one dimensional oscillation of the Belousov-Zhabotinsky reaction confined within microchannels. RSC Advances, 2(16), 6408-6410. Abstract.
Gielen F, deMello AJ, Edel JB (2012). Dielectric cell response in highly conductive buffers. Anal Chem, 84(4), 1849-1853. Abstract.  Author URL.
Niu X, Gielen F, Edel JB, deMello AJ (2011). A microdroplet dilutor for high-throughput screening. Nat Chem, 3(6), 437-442. Abstract.  Author URL.
Gielen F, Pereira F, Demello AJ, Edel JB (2010). High-resolution local imaging of temperature in dielectrophoretic platforms. Anal Chem, 82(17), 7509-7514. Abstract.  Author URL.
Niu X, Gielen F, deMello AJ, Edel JB (2009). Electro-coalescence of digitally controlled droplets. Anal Chem, 81(17), 7321-7325. Abstract.  Author URL.
Gielen F, deMello AJ, Cass T, Edel JB (2009). Increasing the trapping efficiency of particles in microfluidic planar platforms by means of negative dielectrophoresis. J Phys Chem B, 113(5), 1493-1500. Abstract.  Author URL.

Conferences

Collins S, Gielen F, van Vliet L, Kaminski G, Hollfelder F, Spring D (2017). Identification and development of small molecule inhibitors of the aggregation of amyloid beta.  Author URL.
Van Vliet LD, Gielen F, Sinha A, Koprowski BT, Edel JB, Niu X, De Mello AJ, Hollfelder F (2014). Droplet-on-demand platform for biochemical screening and drug discovery.  Abstract.
Niu X, Gielen F, DeMello AJ, Edel JB (2009). A hybrid microfluidic chip for digital electro-coalescence of droplets.  Abstract.

Publications by year


In Press

Anagnostidis V, Sherlock B, Metz J, Mair P, Hollfelder F, Gielen F (In Press). Deep learning guided image-based droplet sorting for on-demand selection. and analysis of single cells and 3D cell cultures.  Abstract.  Author URL.  Full text.

2019

Buryska T, Vasina M, Gielen F, Vanacek P, van Vliet L, Jezek J, Pilat Z, Zemanek P, Damborsky J, Hollfelder F, et al (2019). Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics. Anal Chem, 91(15), 10008-10015. Abstract.  Author URL.  Full text.
Kleine-Brüggeney H, van Vliet LD, Mulas C, Gielen F, Agley CC, Silva JCR, Smith A, Chalut K, Hollfelder F (2019). Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation. Small, 15(5). Abstract.  Full text.

2018

Gielen F, Colin P-Y, Mair P, Hollfelder F (2018). Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics. Methods Mol Biol, 1685, 297-309. Abstract.  Author URL.  Full text.

2017

Mair P, Gielen F, Hollfelder F (2017). Exploring sequence space in search of functional enzymes using microfluidic droplets. Curr Opin Chem Biol, 37, 137-144. Abstract.  Author URL.  Full text.
Collins S, Gielen F, van Vliet L, Kaminski G, Hollfelder F, Spring D (2017). Identification and development of small molecule inhibitors of the aggregation of amyloid beta.  Author URL.
Gielen F, Butz M, Rees EJ, Erdelyi M, Moschetti T, Hyvonen M, Edel JB, Kaminski CF, Hollfelder F (2017). Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets. ANALYTICAL CHEMISTRY, 89(2), 1092-1101. Author URL.

2016

Gielen F, Hours R, Emond S, Fischlechner M, Schell U, Hollfelder F (2016). Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS). Proc Natl Acad Sci U S A, 113(47), E7383-E7389. Abstract.  Author URL.  Full text.

2015

Chen W, Avezov E, Schlachter SC, Gielen F, Laine RF, Harding HP, Hollfelder F, Ron D, Kaminski CF (2015). A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth. Biophys J, 108(5), 999-1002. Abstract.  Author URL.
Gielen F, Buryska T, Van Vliet L, Butz M, Damborsky J, Prokop Z, Hollfelder F (2015). Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets. Anal Chem, 87(1), 624-632. Abstract.  Author URL.
Colin P-Y, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvönen M, Morgavi DP, Janssen DB, Hollfelder F, et al (2015). Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nat Commun, 6 Abstract.  Author URL.

2014

Van Vliet LD, Gielen F, Sinha A, Koprowski BT, Edel JB, Niu X, De Mello AJ, Hollfelder F (2014). Droplet-on-demand platform for biochemical screening and drug discovery.  Abstract.

2013

Gielen F, van Vliet L, Koprowski BT, Devenish SRA, Fischlechner M, Edel JB, Niu X, deMello AJ, Hollfelder F (2013). A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition. Anal Chem, 85(9), 4761-4769. Abstract.  Author URL.

2012

Hassan SU, Gielen F, Niu X, Edel JB (2012). Controlled one dimensional oscillation of the Belousov-Zhabotinsky reaction confined within microchannels. RSC Advances, 2(16), 6408-6410. Abstract.
Gielen F, deMello AJ, Edel JB (2012). Dielectric cell response in highly conductive buffers. Anal Chem, 84(4), 1849-1853. Abstract.  Author URL.

2011

Niu X, Gielen F, Edel JB, deMello AJ (2011). A microdroplet dilutor for high-throughput screening. Nat Chem, 3(6), 437-442. Abstract.  Author URL.

2010

Gielen F, Pereira F, Demello AJ, Edel JB (2010). High-resolution local imaging of temperature in dielectrophoretic platforms. Anal Chem, 82(17), 7509-7514. Abstract.  Author URL.

2009

Niu X, Gielen F, DeMello AJ, Edel JB (2009). A hybrid microfluidic chip for digital electro-coalescence of droplets.  Abstract.
Niu X, Gielen F, deMello AJ, Edel JB (2009). Electro-coalescence of digitally controlled droplets. Anal Chem, 81(17), 7321-7325. Abstract.  Author URL.
Gielen F, deMello AJ, Cass T, Edel JB (2009). Increasing the trapping efficiency of particles in microfluidic planar platforms by means of negative dielectrophoresis. J Phys Chem B, 113(5), 1493-1500. Abstract.  Author URL.

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