The cardiac dyad signalling proteome and its modulation during cardiac protection - PhD in Physics Studentship (BBSRC SWBio DTP funded) Ref: 3977
About the award
Christian Soeller, College of Engineering, Mathematics and Physical Sciences, University of Exeter
Derek Blake, Professor of Neuroscience, University of Cardiff
Location: Streatham/St Luke's Campus, University of Exeter, Exeter, Devon
The SWBio DTP is one of the 12 Doctoral Training Partnerships funded by the BBSRC to provide PhD training in areas of their strategic relevance. The SWBio DTP is a consortium comprising the Universities of Bristol (lead), Bath, Cardiff, Exeter, and Rothamsted Research. Together, these institutions present a distinctive cadre of bioscience research staff and students with established international, national and regional networks and widely recognised research excellence.
The aim of this DTP is to produce highly motivated and excellently trained postgraduates in the BBSRC priority areas of Agriculture & Food Security (AFS) and World-Class Underpinning Bioscience (WCUB). These are growth areas of the biosciences and for which there will be considerable future demand.
This project is one of a number that are in competition for funding from the South West Biosciences Doctoral Training Partnership (SWBio DTP).
Increasing evidence demonstrates that perturbation of a process in heart muscle called excitationcontraction coupling leads to cardiac dysfunction. Excitation-contraction coupling is the name given to the process of molecular events that translates the electrical signal, ‘the heartbeat’, that arrives on the cell membrane in the form of the action potential, into the release of intracellularly stored calcium into the cytoplasm of the heart cell. This process is vital because calcium activates contraction of heart cells to expel blood from the chambers of
the heart and pump it through the circulation. Critical to excitationcontraction coupling are cardiac dyads, membrane microdomains at contact sites between the cell membrane and the sarcoplasmic reticulum (a specialisation of the endoplasmic reticulum) membrane.
Recently, a molecular study has identified proteins that interact at the dyad and form the “dyad proteome”. Based on this finding we will investigate the dyad proteome in health and protection from disease arising from overexpression of a key dyad protein. We will test the hypothesis that maintaining integrity of protein complexes in the dyad proteome is the basis of protection. In this project a number of new molecular tools as well as novel
insights into protein-protein interactions (PPI) in heart cells are coming together to create qualitatively new understanding. In addition to Ca2+-signalling, components of the dyad proteome are involved in controlling membrane curvature, protein phosphorylation and lipid interactions all of which will be studied by super-resolution microscopy.
Supervisor CS laboratory has recently introduced a new super-resolution imaging method to detect and localise PPI in situ. Co-supervisor DB discovered and first described a key protein-complex in the dyad proteome, the myospryn complex. Using the combined approaches in their laboratories we will use the insitu PPI imaging approach to quantitatively determine the changes in the dyad proteome in a mouse model of heart failure and the effect of protection with the support of international collaborator Xander Wehrens (XW). XW has used overexpression of the protein junctophilin-2 (JPH2) to protect against heart failure in a mouse model.
The new approach will map the changes in the dyad proteome for the first time and provide the first comprehensive molecular view of the role of PPIs in maintaining normal cardiac physiology. Successful application of the multidisciplinary approaches to this important aspect of cardiovascular health has the potential to generate high impact outputs and will for a form a strong skill base for the candidate’s further career.
To be eligible for a fully-funded studentship, you must meet both the academic and residence criteria.
A fully-funded four year SWBio DTP studentship will cover
• a stipend* at the standard Research Council UK rate; currently £15,285 per annum for 2020-2021
• research and training costs
• tuition fees (at the standard Research Councils UK rate)
• additional funds to support fieldwork, conferences and a 3-month internship
Please refer to the regulations or Annex 1 of the Research Council Training Grant Guide to confirm that you meet the residence criteria for a fully-funded studentship. Any further queries in relation to residency must be directed to the institution that you are applying to.
* An enhanced stipend is available for students with a recognised veterinary degree qualification (£23,164 per annum for 2019-2020). There may also be enhanced stipends associated with projects that have a CASE partner (CASE projects are highlighted as *CASE in the project lists).
UKRI have recently updated their policy for funded studentships which starts from the 2021/2022 academic year.
IMPORTANT: Please refer to the UKRI Full Eligibility Criteria alongside the information provided below.
Fully-funded studentships are available for Home students. To be classed as a Home student, candidates must meet the following criteria:
• Be a UK National (meeting residency requirements), or
• Have settled status, or
• Have pre-settled status (meeting residency requirement) or,
• Have indefinite leave to remain or enter
If you do not meet the above criteria, you will be classed as an International student.
A limited number (up to 30%) of UKRI fully-funded studentships are available through the SWBio DTP, that applicants who would be classed as an International student are eligible for. Please apply through our normal application route.
Applicants should have obtained, or be about to obtain, a First or Upper Second Class UK Honours degree, or the equivalent qualifications gained outside the UK, in an appropriate area of science or technology. Applicants with a Lower Second Class degree will be considered if they also have Masters degree or have significant relevant non-academic experience.
In addition, due to the strong mathematical component of the taught course in the first year and the quantitative emphasis in our projects, a minimum of a grade B in A-level Maths or an equivalent qualification or experience is required.
If English is not your first language you will need to have achieved at least 6.5 in IELTS and no less than 6.5 in any section by the start of the project. Alternative tests may be acceptable, please see http://www.bristol.ac.uk/study/language-requirements/profile-c/.
- Physics A-level (grade B and above)
- Undertaking units as part of your degree that have a significant mathematical component*
*Significant mathematical component examples include; maths, statistics, bioinformatics.
Applicants must ensure they highlight their Maths background within their application and to upload any supporting evidence.
How to apply
Please be aware you will be asked to upload the following documents:
- Letter of application outlining your academic interests, prior research experience and reasons for wishing to undertake the project. Please indicate your preferred project choice if applying for multiple BBSRC SWBio DTP projects.
- Transcript(s) giving full details of subjects studied and grades/marks obtained. This should be an interim transcript if you are still studying.
- Two academic referees - see information below about references.
- If you are not a national of a majority English-speaking country you will need to submit evidence of your proficiency in English (see entry requirements above)
The closing date for applications is midnight on Monday, 7 December 2020. Interviews will be held at the University of Exeter between w/c 25 January - w/c 1st February 2021.
You will be asked to name 2 referees as part of the application process, however we will not expect receipt of references until after the shortlisting stage. Your referees should not be from the prospective supervisory team.
If you are shortlisted for interview, please ensure that your two academic referees email their references to the firstname.lastname@example.org, 7 days prior to the interview dates. Please note that we will not be contacting referees to request references, you must arrange for them to be submitted to us by the deadline.
References should be submitted by your referees to us directly in the form of a letter. Referees must email their references to us from their institutional email accounts. We cannot accept references from personal/private email accounts, unless it is a scanned document on institutional headed paper and signed by the referee.
If you have any general enquiries about the application process please email email@example.com.
Project-specific queries should be directed to the primary supervisor.
Please note, the studentship selection process will take place in two stages:
1. The project supervisors will consider your application and may invite you to visit for an informal interview. You can apply for more than one BBSRC SWBio DTP project, although supervisors may take into account your interest and commitment to their particular project. If you apply for multiple projects, please indicate your preferred project choice in your letter of application. Each application for an individual project will be considered separately by the project supervisors.
2. After closure of applications, each supervisory team will then nominate their preferred applicant. A shortlist will be selected from these nominations and shortlisted applicants will be invited for interview on a selection day at the University of Bristol. Please note that nomination by a project supervisor therefore does not guarantee the award of a studentship.
During the application process, the University may need to make certain disclosures of your personal data to third parties to be able to administer your application, carry out interviews and select candidates. These are not limited to, but may include disclosures to:
the selection panel and/or management board or equivalent of the relevant programme,
which is likely to include staff from one or more other HEIs;
- administrative staff at one or more other HEIs participating in the relevant programme.
Such disclosures will always be kept to the minimum amount of personal data required for the specific purpose. Your sensitive personal data (relating to disability and race/ethnicity) will not be disclosed without your explicit consent.
|Application deadline:||7th December 2020|
|Value:||Tuition fees and an annual stipend allowance at Research Council rates, currently £15,285 per year for 2020-21|
|Duration of award:||per year|
|Contact: PGR Recruitment Officefirstname.lastname@example.org|