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Dr Ge Guo

Senior Research Fellow

 +44 (0) 1392 72 3042

 Living Systems Institute T02.10

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

Overview

My research is focussed on mammalian pluripotent stem cells and cell fate transition during early embryo development. Key research areas in the lab include:

  1.  Understanding the developmental plasticity of human naïve stem cells.
  2.  Modeling early human embryo development ex vivo by reconstruction of embryo structures.
  3.  Establishing pluripotent stem cells from various mammalian species and elucidation of shared and distinct gene regulatory features (collaborative with Professor Austin Smith)

We are a newly established group seeking enthusiastic researchers to work together.  We have postdoc and potential PhD positions available.  If you are interested in pluripotency and human development please do contact us.

 

 

 

 

Research

Research interests

My main research is centred on human naïve pluripotency and cell fate transition. During my post-doc research I have developed methods to establish human naïve pluripotent stem cells from embryos, conventional human stem cells and from somatic cells. Importantly,  the human naïve pluripotent stem cells we generated are closely related to resident cells in the early human embryo and are genetically stable. My current research shows that unlike mouse embryonic stem cells, human naïve stem cells have the unique potential to differentiate into all cell types in an embryo including the extra-embryonic lineages.  My research group will continue the study on human naïve pluripotency to understand the developmental plasticity and the underlying regulatory mechanisms. Furthermore, by harnessing the unique extraembryonic differentiation potential we aim to establish a synthetic human blastocyst model. This will enable application of the latest molecular, genetic and biophysical approaches to gain knowledge and understanding of human-specific features of early embryo development. 

Key publications

Guo G*., Stirparo J., Strawbridge S., Yang J., Clarke J. , Li M. , Myers S. , Ozel, B, Nichols J., Smith A., Human naive epiblast cells possess unrestricted lineage potency. BioRxiv, preprint (https://doi.org/10.1101/2020.09.04.283218)

Stirparo J.,Smith A. Guo G. Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells. Cell Stem Cell, 28:164-169.e2, (2021)

Bredenkamp, N., Yang, J., Clarke, J., Stirparo, G.G., von Meyenn, F., Dietmann, S., Baker, D., Drummond, R., Ren, Y., Li, D., Wu, C., Rostovskaya, M., Eminli-Meissner, S., Smith, A., Guo, G., 2019. Wnt inhibition facilitates rna-mediated reprogramming of human somatic cells to naive pluripotency. Stem Cell Reports.

Bredenkamp N, Stirparo G.G., Nichols J, Smith A, and Guo G. The cell-surface marker sushi containing domain 2 facilitates establishment of human naive pluripotent stem cells. Stem Cell Reports 12, 1212-1222 (2019).

Stirparo GG, Boroviak T, Guo G, Nichols J, Smith A, Bertone P. Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human pre-implantation epiblast. Development 145(3):07 Feb 2018

Guo G*, Meyenn F, Rostovskaya M, Clarke J, Dietmann S, Baker D, Sahakyan A, Myers S, Bertone P, Reik W, Plath K, Austin Smith. Epigenetic resetting of human pluripotency. Development. 144: 2748-2763. (2017). *Co-corresponding

Guo G, Meyenn F, Santos F, Chen Y, Reik W, Bertone P, Smith A and Nichols J. Naïve pluripotent stem cells derived directly from isolated cells of the human inner cell mass. Stem Cell Reports 6: 437-46 (2016).

Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell 158: 1254-69 (2014).

Research projects

LSI Interdisciplinary PhD Programme Project

Bioengineering naïve stem cell self-organisation to recapitulate the first steps in human embryo development

Supervisor 1: Ge Guo

Supervisor 2: Stefano Pagliara

Project outline

This project is a fusion of stem cell biology with bioengineering and physics of living systems. Human naïve stem cells have the capacity to produce all types of cells including extra embryonic lineages. In this project we aim to control precisely the first differentiation steps so as to produce a synthetic blastocyst comprising three distinct lineages in a specific topological organisation. To achieve this a combination of chemical and environmental cues will be applied to stem cells confined within micro wells in a microfluidic device. This will permitt automated handling of the synthetic blastocysts. The similarity of the synthetic blastocysts to in utero embryos will be evaluated by morphometric and molecular criteria. Further developmental potential will be investigated by extended culture. Finally, the effects of genetic and environmental perturbations on the self-organisation process will be interrogated by real-time imaging and single cell ‘omics.

Publications

Key publications | Publications by category | Publications by year

Publications by category


Journal articles

Stirparo GG, Smith A, Guo G (2021). Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells. Cell Stem Cell, 28(1), 164-169.e2. Abstract.  Author URL.
Guo G, Stirparo GG, Strawbridge SE, Spindlow D, Yang J, Clarke J, Dattani A, Yanagida A, Li MA, Myers S, et al (2021). Human naive epiblast cells possess unrestricted lineage potential. Cell Stem Cell Abstract.  Author URL.
Bredenkamp N, Yang J, Clarke J, Stirparo GG, von Meyenn F, Dietmann S, Baker D, Drummond R, Ren Y, Li D, et al (2019). Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency. Stem Cell Reports, 13(6), 1083-1098. Abstract.  Author URL.
Stirparo GG, Boroviak T, Guo G, Nichols J, Smith A, Bertone P (2018). Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human pre-implantation epiblast. Development, 145(3), dev158501-dev158501.
Stirparo GG, Boroviak T, Guo G, Nichols J, Smith A, Bertone P (2018). Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human pre-implantation epiblast. DEVELOPMENT, 145(3). Author URL.
Guo G, von Meyenn F, Rostovskaya M, Clarke J, Dietmann S, Baker D, Sahakyan A, Myers S, Bertone P, Reik W, et al (2017). Epigenetic resetting of human pluripotency. DEVELOPMENT, 144(15), 2748-2763. Author URL.
Guo G, von Meyenn F, Santos F, Chen Y, Reik W, Bertone P, Smith A, Nichols J (2016). Naive Pluripotent Stem Cells Derived Directly from Isolated Cells of the Human Inner Cell Mass. STEM CELL REPORTS, 6(4), 437-446. Author URL.
Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, et al (2014). Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human. CELL, 158(6), 1254-1269. Author URL.

Publications by year


2021

Stirparo GG, Smith A, Guo G (2021). Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells. Cell Stem Cell, 28(1), 164-169.e2. Abstract.  Author URL.
Guo G, Stirparo GG, Strawbridge SE, Spindlow D, Yang J, Clarke J, Dattani A, Yanagida A, Li MA, Myers S, et al (2021). Human naive epiblast cells possess unrestricted lineage potential. Cell Stem Cell Abstract.  Author URL.

2020

Guo G, Stirparo GG, Strawbridge S, Yang J, Clarke J, Li MA, Myers S, Özel BN, Nichols J, Smith A, et al (2020). Trophectoderm Potency is Retained Exclusively in Human Naïve Cells.  Abstract.

2019

Bredenkamp N, Yang J, Clarke J, Stirparo GG, von Meyenn F, Dietmann S, Baker D, Drummond R, Ren Y, Li D, et al (2019). Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency. Stem Cell Reports, 13(6), 1083-1098. Abstract.  Author URL.

2018

Stirparo GG, Boroviak T, Guo G, Nichols J, Smith A, Bertone P (2018). Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human pre-implantation epiblast. Development, 145(3), dev158501-dev158501.
Stirparo GG, Boroviak T, Guo G, Nichols J, Smith A, Bertone P (2018). Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human pre-implantation epiblast. DEVELOPMENT, 145(3). Author URL.

2017

Guo G, von Meyenn F, Rostovskaya M, Clarke J, Dietmann S, Baker D, Sahakyan A, Myers S, Bertone P, Reik W, et al (2017). Epigenetic resetting of human pluripotency. DEVELOPMENT, 144(15), 2748-2763. Author URL.

2016

Guo G, von Meyenn F, Santos F, Chen Y, Reik W, Bertone P, Smith A, Nichols J (2016). Naive Pluripotent Stem Cells Derived Directly from Isolated Cells of the Human Inner Cell Mass. STEM CELL REPORTS, 6(4), 437-446. Author URL.

2014

Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, et al (2014). Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human. CELL, 158(6), 1254-1269. Author URL.

Ge_Guo Details from cache as at 2021-04-18 09:06:40

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