Technologies
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Flow cytometers
At EXCC, we possess two Aurora spectral analysers that are capable of high dimensional flow cytometry, as well as a Attune NxT for high throughput analysis. We also have an Aurora spectral analyser based at the Environmental and Sustainability Institute in Penryn.
- 5 lasers, 64 fluorescence and 3 Scatter channels
- Enhanced Small Particle (ESP) detection through scatter
- Measure 40 colours simultaneously
- Autofluorescence correction
Applications: Identification of cells, Functional analysis of cells, microbiology, marine and freshwater research, environmental analysis, small particle analysis.
» More about the Aurora 1
- 5 lasers, 64 fluorescence and 3 scatter channels
- Automated Sample Loader (ASL) for high throughput
- Measure 40 colours simultaneously
- Autofluorescence correction
Applications: Identification of cells, Functional analysis of cells, microbiology, marine and freshwater research, environmental analysis, small particle analysis, high throughput screening
» More about the Aurora
- 5 lasers, 14 fluorescence and 2 scatter channels
- Plate loader for high throughput
- Acoustic focusing positions cells in central axis to enable higher sensitivity
- High flow rate to rapidly analyse samples
- Measure up to 14 colours simultaneously
Applications: Identification of cells, Functional analysis of cells, microbiology, marine and freshwater research, environmental analysis, small particle analysis, high throughput screening.
» More about the Attune NxT
- 7 lasers, 21 fluorescence and 2 scatter channels
- Plate loader for high throughput
- Measure up to 21 colours simultaneously
» More about the Cytoflex LX
Imaging cytometers
We have 2 flow cytometry imaging platforms that combine the speed of a flow cytometer with functionality of a microscope, as well as a benchtop fluorescence microscope.
- High resolution imaging of 1000 cells per second
- 5 lasers, allowing 10 fluorescent channels, side-scatter and brightfield images per cell
- Advanced software for quantification of fluorescence and morphological parameters
Applications: Identification of cells, Functional analysis of cells, microbiology, marine and freshwater research, environmental analysis, small particle analysis
» More about the ImageStreamX MKII
- Combine speed of a flow cytometer with functionality of a microscope
- Brightfield color images with 488 nm laser excitation
- Image objects ranging from 2 μm to 1 mm
Applications: Microbiology, Marine and freshwater research, Environmental Water Quality Assessment
» More about the FlowCam 8400
- Digital inverted benchtop fluorescence microscope
- User-defined 4-color fluorescence, transmitted light, and color images
Applications: Identification of cells, functional analysis of cells
» More about the Evos M5000
Cell sorters
EXCC possesses several cell sorters to purify cells from heterogeneous mixtures. This allow the purification of cells for downstream applications from cells isolated from tissues such as for metabolic analysis, in vitro assays, omic measurements or single cell genomic approaches such as a single cell RNA sequencing.
- 6 lasers, 56 Fluorescence, 5 Scatter
- Integrated biocontainment
- Small Particle detector
- Multi-way sorting (up to 6-way, virtually 18-way)
Sort into 96-, 384- and 1536 well plates, petri dishes, microscope slides, and 10x chips
» More about the Bigfoot Spectral Sorter
- 4Laser, 16 Fluorescence, 2 scatter
- BSLII Biosafety Cabinet
- Multi-way sorting (up to 4-way)
Sort into 96-well plates, petri dish, microscope slides, and 10x chips
» More about the BD FACSAria Fusion
Omics
We have multiple platforms to enable omics-based measurements of RNA, protein and other biological molecules.
Microfluidic system to partition cells into individual droplets, where RNA molecules are prepared for sequencing
Prepare hundreds to tens of thousands of cells per run
Applications: Omics technology, Identification of cells, Functional analysis of cells
» More about the Chromium 10X
Performs nucleic acid assays, immunoassays, receptor-ligand assays, and enzymatic assays
96- and 384-well formats
Applications: Screening platform
» More about the Luminex FlexMap 3D
Tissue Cytometry
Tissue cytometry enables high parameter analysis of cells within their tissue context.
- High-resolution fluorescence imaging
- High-content characterisation with up to 400 markers for same tissue slice
Applications: Identification of cells, Location of cells in tissues, Functional analysis of cells
» More about the MACSima